In 1984, Macchio scored the lead role in the "The Karate Kid" (1984) directed by "Rocky" (1976) director John G. Avildsen. The film was a phenomenal success, being highly popular with adults and children alike. The movie spawned two equally popular sequels "The Karate Kid, Part II" (1986) and "The Karate Kid, Part III" (1989), both again starring Macchio and Pat Morita, and both directed by Avildsen.

Macchio also starred in the blues road movie "Crossroads" (1986), featured alongside Joe Pesci in "My Cousin Vinny" (1992) and, looking to toughen up his image, Macchio played a hit man in "A Good Night to Die" (2003). Arguably, movie audiences still identify Macchio very strongly with his "Karate Kid" role, but as his features have gained a more weathered, adult edge, he has found opportunities and positive reviews from appearances in stage productions showcasing his acting talent.